anti-FZD1 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-Fzd1 antibody; Fzd1;
The FZD1 fzd1 (Catalog #MBS629125) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FZD1, ID (Frizzled-1, Fz-1, hFz1, FzE1) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s FZD1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC).Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:10-1:50. Researchers should empirically determine the suitability of the FZD1 fzd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The FZD1 fzd1 product has the following accession number(s) (GI #4503825) (NCBI Accession #NP_003496.1) (Uniprot Accession #Q9UP38). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the FZD1, ID (Frizzled-1, Fz-1, hFz1, FzE1) with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Hela cell line lysates (35ug/lane) using MBS629125. FZD1(arrow) was detected using MBS629125.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human prostata carcinoma tissue using MBS629125, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry.)
Flow Cytometry (FC/FACS) (Flow cytometric analysis of NCI-H292 cells using MBS629125 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Members of the \'frizzled\' family are 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD1 protein contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, 7 transmembrane domains, and a C-terminal PDZ domain-binding motif.
Immunogen: Synthetic peptide selected from the center region of human FZD1 (KLH). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing FZD1 are readily searchable from our website. Different antibodies against the same target such as FZD1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. FZD1 also interacts with the following gene(s): DKK1, LRP5, LRP6, WNT10A, WNT2, WNT3A, WNT5A, WNT7B. The following patways have been known to be associated with this gene.