|The GADD45A gadd45a (Catalog #MBS127973) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GADD45A Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s GADD45A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200. Researchers should empirically determine the suitability of the GADD45A gadd45a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The GADD45A gadd45a product has the following accession number(s) (GI #120751) (NCBI Accession #P24522.1) (Uniprot Accession #P24522). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the GADD45A Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of mouse liver, using GADD45A antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.)
This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human GADD45A
Calculated Molecular Weight: 18kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing GADD45A are readily searchable from our website. Different antibodies against the same target such as GADD45A may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Bone, Brain, Embryonic Tissue, Liver, Lung, Muscle, Prostate, Skin, Vascular tissues are correlated with this protein. The following patways have been known to be associated with this gene. GADD45A also interacts with the following gene(s): CCNB1, CDK1, CDKN1A, FOXO4, GADD45B, GADD45G, MAP2K6, MAP3K4, PCNA, TP53.