anti-GAPDH antibody product blog
Tags: Antibody; GAPDH; anti-GAPDH antibody; Polyclonal Antibody;
The GAPDH gapdh (Catalog #MBS415714) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GAPDH Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s GAPDH can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB).Peptide ELISA titer: 1:20,000-1:80,000
Western blot analysis: 1:500-1000
Optimal dilutions/concentrations should be determined by the end user. Researchers should empirically determine the suitability of the GAPDH gapdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The GAPDH gapdh product has the following accession number(s) (GI #378404906) (NCBI Accession #NP_002037.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-GAPDH Antibody with the following immunoassay(s):
Western Blot (WB)
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014].
Storage Buffer: PBS, pH 7.4 with 0.02% Sodium Azide. Immunogen: Synthetic peptide conjugated to KLH. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing GAPDH are readily searchable from our website. Different antibodies against the same target such as GAPDH may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Brain Diseases, Cardiovascular Diseases, Heart Diseases, Inflammation, Kidney Diseases, Liver Diseases, Liver Neoplasms, Lung Neoplasms, Necrosis, Nervous System Diseases are some of the diseases may be linked to Anti-GAPDH Antibody.