anti-GBE1 antibody product blog
Tags: Antibody; Polyclonal Antibody; Gbe1; anti-GBE1 antibody;
The GBE1 n/a (Catalog #MBS6000399) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GBE1, ID (GBE1, 1,4-alpha-glucan-branching enzyme, Brancher enzyme, Glycogen-branching enzyme) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s GBE1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF).Suitable for use in Western Blot, Immunofluorescence, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunofluorescence: 1:10-50. Researchers should empirically determine the suitability of the GBE1 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The GBE1 n/a product has the following accession number(s) (GI #33621357) (NCBI Accession #AAQ23216.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the GBE1, ID (GBE1, 1,4-alpha-glucan-branching enzyme, Brancher enzyme, Glycogen-branching enzyme) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of GBE1 Antibody (Center) in HL-60 cell line lysates (35ug/lane). GBE1 (arrow) was detected using the purified Pab;)
Western Blot (WB) (Western blot analysis of GBE1 Antibody (Center) in mouse liver tissue lysates (35ug/lane).GBE1 (arrow) was detected using the purified Pab.)
Immunofluorescence (IF) (Confocal immunofluorescent analysis of GBE1 Antibody (Center) with HepG2 cell followed by Alexa Fluor? 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
GBE1 is a glycogen branching enzyme that catalyzes the transfer of alpha-1,4-linked glucosyl units from the outer end of a glycogen chain to an alpha-1,6 position on the same or a neighboring glycogen chain. Branching of the chains is essential to increase the solubility of the glycogen molecule and, consequently, in reducing the osmotic pressure within cells. Highest level of this enzyme are found in liver and muscle. Mutations in this gene are associated with glycogen storage disease IV (also known as Andersen\'s disease).
Immunogen: GBE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 534~564 amino acids from the Central region of human GBE1. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing GBE1 are readily searchable from our website. Different antibodies against the same target such as GBE1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.