anti-GCK antibody product blog
Tags: Antibody; Polyclonal Antibody; GCK; anti-GCK antibody;
The GCK gck (Catalog #MBS129706) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GCK Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s GCK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100. Researchers should empirically determine the suitability of the GCK gck for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The GCK gck product has the following accession number(s) (GI #547696) (NCBI Accession #P35557.1) (Uniprot Accession #P35557). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the GCK Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using GCK antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.)
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).
Species: Human
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human GCK
Calculated Molecular Weight: 52kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing GCK are readily searchable from our website. Different antibodies against the same target such as GCK may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Adipose Tissue, Brain, Embryonic Tissue, Eye, Kidney, Liver, Lung, Lymph Node, Pancreas, Placenta tissues are correlated with this protein. GCK also interacts with the following gene(s): GCKR, GPI, INS, PKLR, SREBF1.