anti-GGA1 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-GGA1 antibody; GGA1;
The GGA1 gga1 (Catalog #MBS646949) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GGA1, NT (GGA1, ADP-ribosylation factor-binding protein GGA1, Gamma-adaptin-related protein 1, Golgi-localized, gamma ear-containing, ARF-binding protein 1) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s GGA1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF).Suitable for use in Western Blot, Immunofluorescence, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunofluorescence. Researchers should empirically determine the suitability of the GGA1 gga1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The GGA1 gga1 product has the following accession number(s) (GI #47678517) (NCBI Accession #CAG30379.1) (Uniprot Accession #Q6IC75). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the GGA1, NT (GGA1, ADP-ribosylation factor-binding protein GGA1, Gamma-adaptin-related protein 1, Golgi-localized, gamma ear-containing, ARF-binding protein 1) with the following immunoassay(s):
Western Blot (WB) (The anti-GGA1 Pab is used in Western blot to detect GGA1 in mouse kidney tissue lysate (lane 1) and Jurkat cell lysate (lane 2).)
Testing Data (A, N2a cells cotransfected with APP770 V5 and GGA1 myc or empty vector were immunostained for APP (Alexa488; green), GGA1 (Cy5; blue), and the Golgi marker GM130 (Cy3; red). B, The same transfection was stained for the endosomal marker EEA1 (Cy3). C, To assess cell-surface localization, APP695 GFP and GGA1 myc or empty vector were cotransfected and then immunostained with an Ab to the APP ectodomain on ice without permeabilization (Cy3). Cells were then fixed, permeabilized, and stained for GGA1 (Cy5). (J. Neurosci. 2006 Sep 27;26(39):9913-9922))
GGA1 is a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) protein family. Members of this family are ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins.
Immunogen: GGA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human GGA1. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing GGA1 are readily searchable from our website. Different antibodies against the same target such as GGA1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.