anti-GLI2 antibody product blog
Tags: Antibody; Polyclonal Antibody; GLI2; anti-GLI2 antibody;
The GLI2 gli2 (Catalog #MBS176275) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Gli2 antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Gli2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the GLI2 gli2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The GLI2 gli2 product has the following accession number(s) (GI #215274258) (NCBI Accession #P10070.4) (Uniprot Accession #P10070). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Gli2 antibody with the following immunoassay(s):
Western Blot (WB) (Anti-Gli2 antibody, MBS176275, Western blotting
WB: Human Placenta Tissue Lysate )
Western Blot (WB) (Anti-Gli2 antibody, MBS176275, Western blotting
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: SKOV Cell Lysate
Lane 4: HT1080 Cell Lysate
Lane 5: A549 Cell Lysate
)
Description: Rabbit IgG polyclonal antibody for Zinc finger protein GLI2(GLI2) detection. Tested with WB in Human.
Background: GLI2(Gli-Kruppel Family Member 2), also called ONCOGENE GLI2, is a protein that in humans is encoded by the GLI2 gene. Sequencing of GLI cDNA clones showed the presence of 5 tandem zinc fingers connected by histidine-cysteine links, which indicated that the gene belongs to the family of zinc finger genes related to Kruppel(Kr). The Drosophila gene Kr is a member of the gap class of segmentation genes; thoracic and anterior abdominal segments fail to form in Kr mutant embryos. By fluorescence in situ hybridization, Matsumoto et al.(1996) refined the assignment of the GLI2 gene to chromosome 2q14. Roessler et al.(2005) showed that GLI2-delta-N exhibited potent transcriptional activity in vivo: overexpression in mouse skin led to the formation of hedgehog-independent epithelial downgrowths resembling basal cell carcinomas, which in humans are associated with constitutive hedgehog signaling.
Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Gli2 (1444-1463aa MLYYYGQIHMYEQDGGLENL). Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing GLI2 are readily searchable from our website. Different antibodies against the same target such as GLI2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.