|The GLI3 gli3 (Catalog #MBS2003923) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to GLI Family Zinc Finger Protein 3 (GLI3) reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s GLI Family Zinc Finger Protein 3 (GLI3) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB).
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the GLI3 gli3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The GLI3 gli3 product has the following accession number(s) (GI #120953173) (NCBI Accession #NP_032156.2) (Uniprot Accession #Q61602). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-SFD LQTMI RTSPNSLVTI LNNSRSSSSA SGSYGHLSAS AISPALSFTY PSAPVSLHMH QQILSRQQSL GSAFGHSPPL IHPAPTFPTQ RPIPGIPTVL NPVQVSSGPS ESSQSKPTSE SAVSSTGDPM HNKRSKIKPD EDLPSPGSRG QQEQPEGTTL VKEEADKDES KQEPEVIYET NCHWEGCTRE FDTQDQLVHH INNDHIHGEK KEFVCRWLDC SREQKPFKAQ YMLVVHMRRH TGEKPHKCTF EGCTKAYSRL E.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Polyclonal Antibody to GLI Family Zinc Finger Protein 3 (GLI3) with the following immunoassay(s):
Western Blot (WB) ((Figure. Western Blot; Sample: Recombinant GLI3, Mouse.))
Immunohistochemistry (IHC) ((Figure. DAB staining on IHC-P; Samples: Mouse Vas Deferens Tissue.))
Immunohistochemistry (IHC) ((Figure. DAB staining on IHC-P; Samples: Mouse Colon Tissue.))
Immunogen: Recombinant GLI3 (Ser303~Glu561) expressed in E.coli. Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2081674. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing GLI3 are readily searchable from our website. Different antibodies against the same target such as GLI3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Brain Diseases, Carcinoma, Chromosome Aberrations, Disease Models, Animal, Glioma, Greig cephalopolysyndactyly syndrome, Neoplasms, Nervous System Diseases, Neural Tube Defects, Pallister-Hall Syndrome are some of the diseases may be linked to Polyclonal Antibody to GLI Family Zinc Finger Protein 3 (GLI3).