|The GM-CSF csf2 (Catalog #MBS695422) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-murine GM-CSF reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s GM-CSF can be used in a range of immunoassay formats including, but not limited to, Neutralization, ELISA (EIA), Western Blot (WB).
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mGM-CSF (0.60 ng/ml), a concentration of 0.012-0.020 ug/ml of this antibody is required.
Sandwich ELISA: To detect mGM-CSF by sandwich ELISA (using 100 mul/well antibody solution) a concentration of 0.5 - 2.0 mug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine GM-CSF as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mGM-CSF.
Western Blot: To detect mGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mGM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Researchers should empirically determine the suitability of the GM-CSF csf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The GM-CSF csf2 product has the following accession number(s) (GI #117562) (NCBI Accession #P01587.1) (Uniprot Accession #P01587). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mGM-CSF. Anti-Murine GM-CSF specific antibody was purified by affinity chromatography employing immobilized mGM-CSF matrix.
Immunogen: Recombinant Murine GM-CSF. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing GM-CSF are readily searchable from our website. Different antibodies against the same target such as GM-CSF may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Arthritis, Rheumatoid, Breast Neoplasms, Carcinoma, Cardiovascular Diseases, Inflammation, Leukemia, Myeloid, Acute, Lung Diseases, Lung Neoplasms, Necrosis, Neoplasms, Experimental are some of the diseases may be linked to Anti-murine GM-CSF. Lung tissues are correlated with this protein.