anti-GNAS antibody product blog
Tags: Antibody; Polyclonal Antibody; GNAS; anti-GNAS antibody;
The GNAS gnas (Catalog #MBS6013420) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GNAS, CT (GNAS) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s GNAS can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF).Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Immunofluorescence: 1:10-50
Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the GNAS gnas for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The GNAS gnas product has the following accession number(s) (GI #30794332) (NCBI Accession #NP_851364.1) (Uniprot Accession #P04896). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the GNAS, CT (GNAS) with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of GNAS (arrow) of 293 cell lysates (2ug/lane) using MBS6013420 using either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GNAS gene.)
Western Blot (WB) (Western Blot analysis in 293,NCI-H292 cell line lysates (35ug/lane) using MBS6013420. This demonstrates that MBS6013420 detected the GNAS protein (arrow).)
Immunohistochemistry (IHC) (Immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue using MBS6013420 followed by peroxidase conjugation of the secondary antibody and DAB staining.)
Immunofluorescence (IF) (Confocal immunofluorescent analysis of 293 cell using MBS6013420 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Flow Cytometry (FC/FACS) (Flow Cytometric analysis of 293 cells using MBS6013420(right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The Gs protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli. Alternative splicing of downstream exons of the GNAS gene is observed, which results in different forms of the stimulatory G protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors.