anti-GPC5 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-GPC5 antibody; GPC5;
The GPC5 gpc5 (Catalog #MBS6012156) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPC5, NT (GPC5, Glypican-5, Secreted glypican-5) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s GPC5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC).Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100. Researchers should empirically determine the suitability of the GPC5 gpc5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The GPC5 gpc5 product has the following accession number(s) (GI #4758464) (NCBI Accession #NP_004457.1) (Uniprot Accession #P78333). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the GPC5, NT (GPC5, Glypican-5, Secreted glypican-5) with the following immunoassay(s):
Western Blot (WB) (GPC5 Antibody (N-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the GPC5 antibody detected the GPC5 protein (arrow).)
Immunohistochemistry (IHC) (GPC5 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GPC5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq].
Immunogen: GPC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-88 amino acids from the N-terminal region of human GPC5. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing GPC5 are readily searchable from our website. Different antibodies against the same target such as GPC5 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Brain, Embryonic Tissue, Kidney, Liver, Lung, Testis tissues are correlated with this protein.