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anti-HAMP antibody product blog

Posted on 2017-04-16 23:57:03 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; HAMP; anti-HAMP antibody; HAMP / Hepcidin;
The HAMP hamp (Catalog #MBS249701) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HAMP / Hepcidin Antibody IHC-plus reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s HAMP / Hepcidin can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB).
IHC-P: 1:200
WB: 1:500 - 1:2000
Optimal dilution to be determined by the researcher. Researchers should empirically determine the suitability of the HAMP hamp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The HAMP hamp product has the following accession number(s) (GI #10863973) (NCBI Accession #NP_066998.1) (Uniprot Accession #P81172). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-HAMP / Hepcidin Antibody IHC-plus with the following immunoassay(s):
Immunohistochemistry - Paraffin (IHC) (Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry - Paraffin (IHC) HAMP.

Western Blot (WB) (Western blot of extracts of MB435 cell lines, using HAMP antibody.)
Western Blot (WB) HAMP.

HAMP Antibody, Hepcidin antimicrobial peptide Antibody, LEAP-1 Antibody, LEAP1 Antibody, HEPC Antibody, Hepcidin Antibody, HFE2B Antibody, PLTR Antibody, Putative liver tumor regressor Antibody Description: Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma.

Immunogen: HAMP / Hepcidin antibody was raised against recombinant full length Human HAMP.
Immunogen Description: Recombinant full length Human HAMP.
Immunogen Type: Recombinant protein
Target: HAMP/Hepcidin. Target Species: Human
Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.

Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60C.

Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

(Do not allow tissues to dry at any time during the staining procedure)
Apply an universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip.
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