anti-HARS2 antibody product blog
Tags: Antibody; Polyclonal Antibody; HARS2; anti-HARS2 antibody;
The HARS2 hars2 (Catalog #MBS9128444) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HARS2 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s HARS2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).WB 1:500 - 1:2000
IHC 1:50 - 1:200. Researchers should empirically determine the suitability of the HARS2 hars2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The HARS2 hars2 product has the following accession number(s) (GI #523567531) (NCBI Accession #NP_001265660.1) (Uniprot Accession #P49590). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the HARS2 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using HARS2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat brain using HARS2 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse kidney using HARS2 antibody at dilution of 1:100 (40x lens).)
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is an enzyme belonging to the class II family of aminoacyl-tRNA synthetases. Functioning in the synthesis of histidyl-transfer RNA, the enzyme plays an accessory role in the regulation of protein biosynthesis. The gene is located in a head-to-head orientation with HARS on chromosome five, where the homologous genes likely share a bidirectional promoter. Mutations in this gene are associated with the pathogenesis of Perrault syndrome, which involves ovarian dysgenesis and sensorineural hearing loss. Alternative splicing results in multiple transcript variants of this gene.
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human HARS2. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing HARS2 are readily searchable from our website. Different antibodies against the same target such as HARS2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.