anti-HBA1 antibody product blog
Tags: Antibody; Polyclonal Antibody; HBA2; anti-HBA1 antibody; HBA1;
The HBA1 hba1 (Catalog #MBS9211991) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HBA2 Antibody (Center) reacts with Human, mouse (Predicted Reactivity: Monkey) and may cross-react with other species as described in the data sheet. MyBioSource\'s HBA2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS).WB~~1:1000. Researchers should empirically determine the suitability of the HBA1 hba1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The HBA1 hba1 product has the following accession number(s) (GI #4504345) (NCBI Accession #NP_000508.1) (Uniprot Accession #P69905). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the HBA2 Antibody (Center) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of HBA2 Antibody (Center) in mouse liver tissue lysates (35ug/lane). HBA2 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HBA2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (HBA2 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
HBA2 located on chromosome 16 spans about 30 kb and includes seven loci: 5\'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3\'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5\' untranslated regions and the introns, but they differ significantly over the 3\' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.
Antigen Type: Synthetic Peptide. Antigen Source: HUMAN. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing HBA1 are readily searchable from our website. Different antibodies against the same target such as HBA1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.