anti-HCN3 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-HCN3 antibody; HCN3;
The HCN3 hcn3 (Catalog #MBS612134) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HCN3 (Hyperpolarization-activated Cyclic Nucleotide-gated channel 3) reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s HCN3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1-10ug/ml using ECL.
ELISA: 1:10,000-1:100,000 using 50-100ng of Control Peptide/well. Researchers should empirically determine the suitability of the HCN3 hcn3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The HCN3 hcn3 product has the following accession number(s) (GI #62914013) (Uniprot Accession #Q2T9L6). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Biological rhythms (beating of the heart, circadian sleep cycles, respiration, and the release of hormones) are necessary to sustain life. Heart beating is the most reliable and rhythmic biological phenomenon. Cardiac pacemaking is produced by the slow diastolic depolarization phase of the action potential. The hyperpolarization-activated cation current (termed If, Ih, or Iq) plays a key role in the in the initiation and modulation of cardiac and neuronal pacemaker depolarization. The generation of cardiac pacemaker potentials relies on a complex interplay between at least four different types of cation channels: T- and L-type Ca2+ channels, K+ channels, and a cation channel termed If (synonymous names are Ih and Iq). The If channel has been designated as "pacemaker" channel because it reveals unique features that are believed to be a prerequisite for pacemaker activity. Recently, the hyperpolarization-activated cyclic nucleotide-gated families of ion channel proteins (HCN1-4) have been identified as the "pacemaker" channel. The amino acid sequences of HCNs predict a structure similar to that of voltage-gated (Kv) channels and cyclic nucleotide-gated (CNG) channels. HCNs proteins (HCN1-4) are characterized by six transmembrane domains (S1-S6), including an including a positively charged voltage-sensing S4 segment and an ion-conducting pore between S5 and S6. In the C terminus the HCNs carry a cyclic nucleotide-binding domain (CNBD), a motif found in several cyclic nucleotide-binding proteins. The core region of HCNs channels (S1 to the C terminus of the CNBD) is highly conserved, whereas the cytoplasmic N and C-termini, vary considerably in their length and share only weak sequence homology. The functional HCN, like other members of the superfamily of voltage-gated cation channels, channels probably assemble into tetrameric complexes. HCN3 or HAC3 (rat 780aa, mouse 779aa, human 703aa) has been detected in brain but its expression was much less than other HCNs. Its expression was also detected in liver, lung, and kidney of mouse by some researchers but not by others.