anti-HIF3A antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-HIF3A antibody; HIF3A; HIF3;
The HIF3A hif3a (Catalog #MBS176062) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HIF3 antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s HIF3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the HIF3A hif3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The HIF3A hif3a product has the following accession number(s) (GI #145558932) (NCBI Accession #Q9Y2N7.2) (Uniprot Accession #Q9Y2N7). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-HIF3 antibody with the following immunoassay(s):
Western Blot (WB) (Anti-HIF3 antibody, MBS176062, Western blotting
WB: Rat Brain Tissue Lysate )
Description: Rabbit IgG polyclonal antibody for Hypoxia-inducible factor 3-alpha(HIF3A) detection. Tested with WB in Human, Rat.
Background: Hypoxia-inducible factor 3 alpha, also called MOP7, is a protein that in humans is encoded by the HIF3A gene. HIF3A is mapped to 19q13.32. This gene involved in adaptive response to hypoxia. HIF3A suppresses hypoxia-inducible expression of HIF1A and EPAS1. It binds to core DNA sequence 5\'-TACGTG-3\' within the hypoxia response element(HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. This gene functions as an inhibitor of angiogenesis in the cornea.
Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human HIF3 (497-514aa DDDFQLNASEQLPRAYHR), different from the related rat and mouse sequences by five amino acids. Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing HIF3A are readily searchable from our website. Different antibodies against the same target such as HIF3A may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. HIF3A also interacts with the following gene(s): ARNT, HIF1A, VHL.