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anti-HIST1H2BG antibody product blog

Posted on 2015-07-24 17:18:15 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; Histone H2B type 1-C/E/F/G/I; anti-HIST1H2BG antibody; HIST1H2BG;
The HIST1H2BG hist1h2bc (Catalog #MBS715401) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Histone H2B type 1-C/E/F/G/I polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Histone H2B type 1-C/E/F/G/I can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Researchers should empirically determine the suitability of the HIST1H2BG hist1h2bc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The HIST1H2BG hist1h2bc product has the following accession number(s) (GI #4504257) (NCBI Accession #NP_003509.1) (Uniprot Accession #P62807). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit anti-human Histone H2B type 1-C/E/F/G/I polyclonal Antibody with the following immunoassay(s):
Immunohistochemistry (IHC) (IHC image of MBS715401 diluted at 1:600 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC) HIST1H2BG.

Immunofluorescence (IF) (Immunofluorescence staining of Hela cells with MBS715401 at 1:200,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Immunofluorescence (IF) HIST1H2BG.

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.

Storage Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugate: Non-conjugated. Immunogen: Recombinant human Histone H2B type 1-C/E/F/G/I protein.
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