anti-hist1h2bo antibody product blog
Tags: Antibody; Polyclonal Antibody; HIST1H2BO; Histone H2B, acetylated; anti-hist1h2bo antibody;
The hist1h2bo n/a (Catalog #MBS610297) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H2B, acetylated (Lys5) reacts with Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Histone H2B, acetylated can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC).Suitable for use in ELISA, Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin sections) and Immunocytochemistry (ABC detection method).
Dilution: Western Blotting: 1:1000
Immunoprecipitation: 1:50
Immunohistochemistry (Paraffin): 1:100
Immunocytochemistry (ABC): 1:50. Researchers should empirically determine the suitability of the hist1h2bo n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The hist1h2bo n/a product has the following accession number(s) (GI #288684309) (NCBI Accession #NP_001165767.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Histone H2B, acetylated (Lys5) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts from various cell lines, untreated or TSA-treated (400 nM for 12 hours), using Acetyl-Histone H2B (Lys5) Antibody.)
Immunohistochemistry (IHC) (Immunohistochemical staining of paraffin-embedded human breast carcinoma, showing nuclear localization of acetyl-histone H2B, using Acetyl-Histone H2B (Lys5) Antibody.)
Immunocytochemistry (ICC) (Immunocytochemical staining of MCF-7 cells, untreated (left) or TSA-treated (400 nM for 12 hours) (right), using Acetyl-Histone H2B (Lys5) Antibody.)
Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin (1). The N-terminal tail of core histones undergoes different post-translational modifications including acetylation, phosphorylation and methylation (2-4). These modifications occur in.
Immunogen: Synthetic acetyl-Lys5 peptide (KLH coupled) corresponding to residues surrounding Lys5 of human histone H2b. Note: Adherence to the attached protocol is extremely important. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing hist1h2bo are readily searchable from our website. Different antibodies against the same target such as hist1h2bo may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.