anti-HLA-G antibody product blog
Tags: Antibody; HLA-G; Polyclonal Antibody; b2-Microglobulin; anti-HLA-G antibody;
The HLA-G hla-g (Catalog #MBS610505) is an Antibody produced from Host: RabbitSource: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti - b2-Microglobulin (B2M) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s b2-Microglobulin can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot.
Suitable for use in Western Blot and ELISA. Other applications not tested.
Dilution: Western Blot: 1:1000-1:5000 using Chemiluminescence technique. B2M is ~12kD. See M3890-55B for positive control.
ELISA: 1:100,000; using 50-100ng control antigen/well.
Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the HLA-G hla-g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The HLA-G hla-g product has the following accession number(s) (GI #188468) (Uniprot Accession #O02923). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Beta-2-microglobulin (B2M) is found in the serum of normal individuals and in the urine in elevated amounts in patients with Wilson disease, cadmium poisoning, and other conditions leading to renal tubular dysfunction. Like immunoglobulins, prealbumin, and the beta protein found in the amyloid of Alzheimer disease, B2M has a predominantly beta-pleated sheet structure that may adopt the fibrillar configuration of amyloid in certain pathologic states. The protein is a single polypeptide chain of molecular weight 11,600 (human 119 aa, chromosome 15). B2M associates with heavy hcain of class I MHC antigens on the cell surface. A transient complex of MHC-heavy chain and B2M is is known to be assembled into the TAP molecule involving interation with a number of chaperones. Binding of the processed peptide releases the class I-B2M complex to the cell surface. Absence of binding leads to degradation in the proteasome. Progressive hepatic iron overload, indistinguishable from that observed in HFE, was found only in mice homozygous for the mutated B2M gene.
Immunogen: Purified human B2M. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing HLA-G are readily searchable from our website. Different antibodies against the same target such as HLA-G may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.