|The HMGCL hmgcl (Catalog #MBS9128413) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HMGCL Polyclonal Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s HMGCL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).
WB 1:500 - 1:2000
IHC 1:50 - 1:200. Researchers should empirically determine the suitability of the HMGCL hmgcl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The HMGCL hmgcl product has the following accession number(s) (GI #62198232) (NCBI Accession #NP_000182.2) (Uniprot Accession #P35914). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the HMGCL Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using HMGCL antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 15s.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat kidney using HMGCL antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human colon carcinoma using HMGCL antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human kidney using HMGCL antibody at dilution of 1:100 (40x lens).)
The protein encoded by this gene belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associated with HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human HMGCL. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing HMGCL are readily searchable from our website. Different antibodies against the same target such as HMGCL may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Adipose Tissue, Blood, Brain, Connective Tissue, Heart, Kidney, Liver, Muscle, Prostate, Skin tissues are correlated with this protein.