anti-HMOX2 antibody product blog
Tags: Antibody; Polyclonal Antibody; HMOX2; anti-HMOX2 antibody; Heme Oxygenase-2;
The Heme Oxygenase-2 hmox2 (Catalog #MBS840525) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Heme Oxygenase-2 Antibody reacts with Human, Mouse, Rat, Monkey, Hamster and may cross-react with other species as described in the data sheet. MyBioSource\'s Heme Oxygenase-2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP).Western blotting (0.5-4 ug/ml), in immunoprecipitation (20 ug/ml) and Immunohistochemistry (20 ug/ml, frozen section tested). However, the optimal concentrations should be determined individually. The antibody recognizes ~36 kDa Heme-Oxygenase-2 of human, mouse, rat, hamster, and monkey origins. Blocking peptide is available separately. Researchers should empirically determine the suitability of the Heme Oxygenase-2 hmox2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Heme Oxygenase-2 hmox2 product has the following accession number(s) (GI #443771) (NCBI Accession #BAA04789.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Heme Oxygenase-2 Antibody with the following immunoassay(s):
Testing Data
Background: Heme oxygenase (HO) is a microsomal enzyme that oxidatively cleaves heme, a pro-oxidant, into carbon monoxide and biliverdin. Two heme oxygenase isozymes, termed HO-1 and HO-2, have been identified so far. At the amino acid level, HO-1 and HO-2 are 42% homologous. However, each isozyme is evolutionary highly conserved. HO-1 is the inducible form of the enzyme and is therefore also referred to as stress protein Hsp32. Expression of HO-1 can be induced by a variety of stimuli such as heme, metals, hormones, UV radiation and sulfhydryl depleting agents. In contrast, HO-2 is the constituitive form of the enzyme. It is not inducible by exogeneous stimuli and HO-2 is the most prevalent form in most tissues, except the spleen where HO-1 levels are predominant under normal conditions.
Immunogen: Synthetic peptide surrounding amino acid 34 of human HO-2. Handling: The antibody solution should be gently mixed before use. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Heme Oxygenase-2 are readily searchable from our website. Different antibodies against the same target such as Heme Oxygenase-2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.