anti-hnNOS [1411-1433] IgG fraction antibody product blog
Tags: Antibody; Polyclonal Antibody; hnNOS [1411-1433] IgG fraction; anti-hnNOS [1411-1433] IgG fraction antibody;
The hnNOS [1411-1433] IgG fraction n/a (Catalog #MBS352113) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Anti-human neuronal Nitric Oxide Synthase Receptor Serum reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s hnNOS [1411-1433] IgG fraction can be used in a range of immunoassay formats including, but not limited to, Immunocytochemical Staining, Western Immunoblot, Western Blot.Immunocytochemistry: This antiserum has been found to stain fixed cultured cells known to express hnNOS by indirect immunofluorescence at a dilutions of 1:1000 to 1:5000.
Western Blot: Western immunoblots using homogenate from cells expressing hnNOS resulted in a single band being detected at ~ 160 kDa at a dilution of 1:2000.
Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and elecrophoretic transfer to PVDF membrane overnight with 4% normal goat serum in 1:5 diluted evaporated goat milk. using TBS/Tween-20 buffer as diluent.
2. Wash x 2 with TBS/Tween-20.
3. Apply the rabbit polyclonal antibody after dilution to at least 1:800(Note:higher dilutions may be needed). Use 1% normal goat serum in 1:5 diluted evaporated goat milk mild as buffer for the primary antibody. Dilute the condensed goat milk with TBS/Tween-20. Let the primary antibody bind for 2-4 hours.
4. Wash x 3 with TBS/Tween-20.
5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500(dilution may vary depending upon supplier) in 1% normal goat serum in 1:5 diluted evaporated goat milk(useTBS/Tween-20 to dilute the goat milk). Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.
6. Wash x 3 and then soak the membrane overnight in a fairly large volume to TBS/Tween-20.
7. Develop color using the DAB reaction or the enhanced DAB reaction. Researchers should empirically determine the suitability of the hnNOS [1411-1433] IgG fraction n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
The antiserum was raised in a rabbit which was immunized with hnNOS(1411-1433) covalently attached onto a carrier protein. This antiserum is specific for the carboxyl end of hnNOS and is suitable for immunocytochemical and western immunoblotting detection of hnNOS. The antiserum has been characterized by western blotting. ELISA. and cell staining techniques using synthetic peptides and recombinant whole proteins. The IgG fraction of the rabbit antiserum was prepared by precipitation. dialysis. and column chromatography. Dilute the lyophilized antiserum with 0.1 ml of 10 mg/ml BSA is PBS for the equivalent of whole antiserum. or with additional buffer for more dilute antiserum.
In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing hnNOS [1411-1433] IgG fraction are readily searchable from our website. Different antibodies against the same target such as hnNOS [1411-1433] IgG fraction may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.