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anti-HRK antibody product blog

Posted on 2015-08-24 12:56:31 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; Hrk; anti-HRK antibody;
The HRK hrk (Catalog #MBS223071) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s HRK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).
Western Blot: This item detects a band of approximately 13kDa in mouse pancreas tissue lysate.
Western Blotting: Minimum Dilution: 2; Maximum Dilution: 5ug/ml. Researchers should empirically determine the suitability of the HRK hrk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The HRK hrk product has the following accession number(s) (GI #4504493) (NCBI Accession #NP_003797.1) (Uniprot Accession #O00198). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RABBIT ANTI HRK with the following immunoassay(s):
Testing Data #1 (Western blot analysis of mouse pancreas lysate probed with Rabbit anti Human Hrk (MBS221467) at 2.5(A) and 5(B) ug/ml)
Testing Data #1 HRK.

MBS221467 detects Harakiri (HRK), also known as DP5. HRK is a BH3 only protein of the Bcl-2 family, which promotes Bax dependent apoptosis. HRK regulates apoptosis through its interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL, and in conjunction to the mitochondrial pore forming protein p32. HRK is thought to have a specific role in initiating neuronal and haematopoietic cell apoptosis, in particular to growth factor deprivation. Aberrant methylation of HRK is frequently detected in primary colorectal cancers. Despite its predicted molecular weight, HRK often migrates at 12-15kD.

Perservative Stabilisers: 0.02% Sodium Azide. Immunogen: 15 amino acid peptide from near the centre of human HRK (NP_003797).
Buffer Solution: Phosphate buffered saline
Antiserum Preparation: Antisera to HRK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Target Species: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing HRK are readily searchable from our website. Different antibodies against the same target such as HRK may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. HRK also interacts with the following gene(s): BAD, BCL2L1, BIK, BMF, JUN. The following patways have been known to be associated with this gene. Brain Diseases, Breast Neoplasms, Cardiovascular Diseases, Disease Models, Animal, Hepatitis, Inflammation, Mitochondrial Diseases, Necrosis, Nervous System Diseases, Seizures are some of the diseases may be linked to RABBIT ANTI HRK.
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