anti-HSD11B2 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-HSD11B2 antibody; HSD11B2;
The HSD11B2 hsd11b2 (Catalog #MBS9128878) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HSD11B2 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s HSD11B2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).WB 1:200 - 1:1000
IHC 1:20 - 1:200. Researchers should empirically determine the suitability of the HSD11B2 hsd11b2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The HSD11B2 hsd11b2 product has the following accession number(s) (GI #119392083) (NCBI Accession #NP_000187.3) (Uniprot Accession #P80365). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the HSD11B2 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of HepG2 cells, using HSD11B2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 15s.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat kidney using HSD11B2 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human liver injury using HSD11B2 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human stomach using HSD11B2 antibody at dilution of 1:100 (40x lens).)
There are at least two isozymes of the corticosteroid 11-beta-dehydrogenase, a microsomal enzyme complex responsible for the interconversion of cortisol and cortisone. The type I isozyme has both 11-beta-dehydrogenase (cortisol to cortisone) and 11-oxoreductase (cortisone to cortisol) activities. The type II isozyme, encoded by this gene, has only 11-beta-dehydrogenase activity. In aldosterone-selective epithelial tissues such as the kidney, the type II isozyme catalyzes the glucocorticoid cortisol to the inactive metabolite cortisone, thus preventing illicit activation of the mineralocorticoid receptor. In tissues that do not express the mineralocorticoid receptor, such as the placenta and testis, it protects cells from the growth-inhibiting and/or pro-apoptotic effects of cortisol, particularly during embryonic development. Mutations in this gene cause the syndrome of apparent mineralocorticoid excess and hypertension.
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human HSD11B2. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing HSD11B2 are readily searchable from our website. Different antibodies against the same target such as HSD11B2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.