anti-HSD17B13 antibody product blog
Tags: Antibody; Polyclonal Antibody; HSD17B13; anti-HSD17B13 antibody;
The HSD17B13 hsd17b13 (Catalog #MBS9125481) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The HSD17B13 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s HSD17B13 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).WB: 1:500 - 1:2000
IHC: 1:50 - 1:200. Researchers should empirically determine the suitability of the HSD17B13 hsd17b13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The HSD17B13 hsd17b13 product has the following accession number(s) (GI #74750138) (NCBI Accession #Q7Z5P4.1) (Uniprot Accession #Q7Z5P4). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the HSD17B13 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using HSD17B13 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS9125481) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human liver injury using HSD17B13 Antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse liver using HSD17B13 Antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat brain using HSD17B13 Antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human liver injury using HSD17B13 Antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse liver using HSD17B13 Antibody at dilution of 1:100 (40x lens).)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using HSD17B13 antibody (MBS9125481) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human liver using HSD17B13 Rabbit pAb (MBS9125481) at dilution of 1:50 (40xlens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse liver using HSD17B13 Rabbit pAb (MBS9125481) at dilution of 1:50 (40xlens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat liver using HSD17B13 Rabbit pAb (MBS9125481) at dilution of 1:50 (40x lens).)
Source: Rabbit. Immunogen: Recombinant funsion protein containing a sequence corresponding to amino acids 20-300 of human HSD17B13 (NP_835236.2).
Calculated MW: 29 kDa/33 kDa
Observed MW: 25-38 kDa
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing HSD17B13 are readily searchable from our website. Different antibodies against the same target such as HSD17B13 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.