anti-HSP25 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-HSP25 antibody; HSP25; Heat Shock Protein 25;
The HSP25 hsp25 (Catalog #MBS629811) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Heat Shock Protein 25, phosphorylated (Ser86) (HSP25) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Heat Shock Protein 25 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the HSP25 hsp25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The HSP25 hsp25 product has the following accession number(s) (GI #58037558) (NCBI Accession #NP_001010842.1) (Uniprot Accession #Q6BDR9). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Heat Shock Protein 25 (HSP25), is a 25kD member of a family of proteins whose expression and function are stimulated by heat shock and other stress stimuli. A major function of these proteins is to serve as chaperones that bind to and stabilize the active conformation of other proteins. HSP25, along with other members of the small HSP group, possesses a C-terminal alpha-crystalline homology domain. HSP25 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. Cytoplasmic HSP25 exists in multiple complexes. One complex consists of HSP25, Akt (PKB), MAPKAP-kinase 2, and p38 MAPK. The presence of HSP25 in this complex is required for Akt activation by stress stimuli. Another complex consists of HSP25 and the IKK complex. HSP25 is also an actin capping protein that binds to the barbed (growing) ends of actin filaments, thereby inhibiting filament extension. Phosphorylation of HSP25 on serine 86 by MAPKAP-kinase 2 leads to HSP25 dissociation from the Akt/MAPKAP-kinase 2/p38 MAPK complex and from actin filaments, and stimulates HSP25 binding to the IKK complex.
Immunogen: Synthetic phosphpeptide corresponding to the region of mouse HSP25 that contains serine 86. Positive Control: NIH3T3 cells with and without anisomycin. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing HSP25 are readily searchable from our website. Different antibodies against the same target such as HSP25 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.