anti-HSP90B antibody product blog
Tags: Antibody; anti-HSP90B antibody; HSP90B; Polyclonal Antibody;
The HSP90B hsp90ab1 (Catalog #MBS9406391) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HSP90B (Ab-254) Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s HSP90B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500. Researchers should empirically determine the suitability of the HSP90B hsp90ab1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The HSP90B hsp90ab1 product has the following accession number(s) (GI #431822401) (NCBI Accession #NP_001258898.1) (Uniprot Accession #P08238). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the HSP90B (Ab-254) Antibody with the following immunoassay(s):
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP90B (Ab-254) antibody.)
Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cells, treated with TNF-a (20nM, 15mins), using HSP90B (Ab-254) antibody.)
Western Blot (WB) (Western blot analysis of extracts from Hela cells treated with TNF-a (20ng/ml, 30min), using HSP90B (Ab-254) antibody.)
Western Blot (WB) (Western blot analysis of extracts from HeLa cells (Lane 2), CoLo cells (Lane 3), HepG2 cells (Lane 4) and 293 cells (Lane 5), using HSP90B (Ab-254) antiobdy. The lane on the left is treated with synthesized peptide.)
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.Lothar Goretzki, AACR Meeting Abstracts, Apr 2006; 2006: 468.Jean-Fran ois Louvion, Mol. Biol. Cell, Nov 1998; 9: 3071.Angelika Lueking, Mol. Cell. Proteomics, Dec 2003; 2: 1342.Jason Fontana Circ, Res., May 2002; 90: 866.
Immunogen Type: Peptide
Immunogen Description: Synthesized non-phosphopeptide derived from human HSP90B around the phosphorylation site of serine 254 (V-G-S(p)-D-E). Target Name: HSP90B. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing HSP90B are readily searchable from our website. Different antibodies against the same target such as HSP90B may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.