anti-HSPA5 antibody product blog
Tags: Antibody; HSPA5; Polyclonal Antibody; anti-HSPA5 antibody; 78 kDa glucose-regulated;
The HSPA5 hspa5 (Catalog #MBS7002722) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human 78 kDa glucose-regulated polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s 78 kDa glucose-regulated can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC).Recommended dilution: IHC: 1:20-1:200. Researchers should empirically determine the suitability of the HSPA5 hspa5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The HSPA5 hspa5 product has the following accession number(s) (GI #16507237) (NCBI Accession #NP_005338.1) (Uniprot Accession #P11021). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit anti-human 78 kDa glucose-regulated polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western Blot
Positive WB detected in: Hela whole cell lysate,MCF-7 whole cell lysate,HepG2 whole cell lysate,A549 whole cell lysate
All lanes: HSPA5 antibody at 4ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 73 KDa
Observed band size: 73 KDa
)
Immunohistochemistry (IHC) (IHC image of MBS7002722 diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC) (IHC image of MBS7002722 diluted at 1:200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunofluorescence (IF) (Immunofluorescence staining of HepG2 cells with MBS7002722 at 1:66,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.
Immunogen: Recombinant human 78 kDa glucose-regulated protein. Conjugation: Non-conjugated
Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-1051 / sc-1050 / sc-13968 / sc-13539.