anti-HSPH1 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-HSPH1 antibody; HSPH1; Hsp110;
The HSPH1 sept8 (Catalog #MBS805428) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Hsp110 Antibody: HRP reacts with Human, Monkey, Hamster, Rat, Mouse, Cow, Sheep, Yeast and may cross-react with other species as described in the data sheet. MyBioSource\'s Hsp110 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).1:1000 (WB). Researchers should empirically determine the suitability of the HSPH1 sept8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The HSPH1 sept8 product has the following accession number(s) (NCBI Accession #NP_006635.3) (Uniprot Accession #Q92599). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Hsp110 Antibody: HRP with the following immunoassay(s):
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Rabbit Anti-HSP110 Polyclonal Antibody (SPC-195). Tissue: backskin. Species: Mouse. Fixation: Bouin\'s Fixative Solution. Primary Antibody: Rabbit Anti-HSP110 Polyclonal Antibody (SPC-195) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Positive epidermal, dermal and muscle staining.)
Western Blot (WB) (Western blot analysis of Human Cell line lysates showing detection of HSP110 protein using Rabbit Anti-HSP110 Polyclonal Antibody (SPC-195). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP110 Polyclonal Antibody (SPC-195) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)
Background Info: Detects ~110kDa
Scientific Background: Hsp110 belongs to a family of large stress proteins known as the Hsp110/SSE Family. The proteins in this family are the most distantly known relatives of the well studied Hsp70 family. They share 30-33% amino acid identity, mostly in the conserved ATP-binding domain (1). Hsp110 cooperates with Hsp70 in protein folding in the eukaryotic cytosol (2). In mammals, Hsp110 is constitutively expressed, but exhibits particularly high levels in the brain. Both Hsp70 and Hsp110 are elevated after cerebral ischemia. Recent studies demonstrate that the protective effects of Hsp110 deficiency in cerebral ischemia may partly be mediated by an increase in the chaperone activity of Hsp70 (3). Studies also suggest that Hsp110 can be used in heat shock protein-based cancer immunotherapy (4).
Conjugate: HRP. Storage Buffer: PBS pH7.2, 50% glycerol, 0.09% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing HSPH1 are readily searchable from our website. Different antibodies against the same target such as HSPH1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.