|The I kappa B-alpha nfkbia (Catalog #MBS9404605) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The I kappa B-alpha antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s I kappa B-alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF).
Western blotting: 1:500-1:3000
Immunofluorescence: 1:100-1:200. Researchers should empirically determine the suitability of the I kappa B-alpha nfkbia for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The I kappa B-alpha nfkbia product has the following accession number(s) (GI #10092619) (NCBI Accession #NP_065390). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the I kappa B-alpha antibody with the following immunoassay(s):
Testing Data (Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE Primary antibody diluted at 1: 1000)
Immunofluorescence (IF) (Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using IkappaB-alpha antibody at 1: 200 dilution.)
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an An or G purine; and Y is a C or T pyrimidine).[supplied by OMIM].
Immunogen Type: Recombinant protein
Immunogen Description: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 305 of Human NFKBIA. Target Name: I kappa B-alpha. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing I kappa B-alpha are readily searchable from our website. Different antibodies against the same target such as I kappa B-alpha may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Bone, Brain, Heart, Kidney, Liver, Lung, Muscle, Skin, Vascular tissues are correlated with this protein.