anti-IDO1 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-IDO1 antibody; IDO1; INDO;
The IDO1 ido1 (Catalog #MBS126565) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The INDO Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s INDO can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).WB: 1:500 - 1:2000
IHC: 1:50 - 1:200. Researchers should empirically determine the suitability of the IDO1 ido1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The IDO1 ido1 product has the following accession number(s) (GI #123948) (NCBI Accession #P14902.1) (Uniprot Accession #P14902). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the INDO Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using IDO1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.)
INDO/IDO1/indoleamine 2,3-dioxygenase (IDO) is an IFN-gamma-inducible enzyme that catalyzes the rate-limiting step of tryptophan degradation (1). IDO is upregulated in many tumors and in dendritic cells in tumor-draining lymph nodes. Elevated tryptophan catabolism in these cells leads to tryptophan starvation of T cells, limiting T cell proliferation and activation (2). Therefore, IDO is considered an immunosuppresive molecule, and research studies have shown that upregulation of IDO is a mechanism of cancer immune evasion (3). The gastrointestinal stromal tumor drug, imatinib, was found to act, in part, by reducing IDO expression, resulting in increased CD8+ T cell activation and induction of apoptosis in regulatory T cells (4). In addition to its enzymatic activity, IDO was recently shown to have signaling capability through an immunoreceptor tyrosine-based inhibitory motif (ITIM) that is phosphorylated by Fyn in response to TGF-beta. This leads to recruitment of SHP-1 and activatation of the noncanonical NF-kappaB pathway (5).
Species: Human
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human INDO
Calculated Molecular Weight: 45kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing IDO1 are readily searchable from our website. Different antibodies against the same target such as IDO1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.