anti-IGF1R antibody product blog
Tags: Antibody; anti-IGF1R antibody; IGF1R; Polyclonal Antibody;
The IGF1R igf1r (Catalog #MBS646277) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IGF1R, NT (K66) (IGF1R, Insulin-like growth factor 1 receptor, Insulin-like growth factor I receptor, CD221, Insulin-like growth factor 1 receptor alpha chain, Insulin-like growth factor 1 receptor beta chain) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s IGF1R can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF).Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Dilution: ELISA: 1:1,000
Western Blot: 1:50-100
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Immunofluorescence: 1:10-50. Researchers should empirically determine the suitability of the IGF1R igf1r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The IGF1R igf1r product has the following accession number(s) (GI #4557665) (NCBI Accession #NP_000866.1) (Uniprot Accession #P08069). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the IGF1R, NT (K66) (IGF1R, Insulin-like growth factor 1 receptor, Insulin-like growth factor I receptor, CD221, Insulin-like growth factor 1 receptor alpha chain, Insulin-like growth factor 1 receptor beta chain) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of IGF1R Antibody (N-term K66) in WiDr cell line lysates (35ug/lane).IGF1R (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (IGF1R Antibody (N-term K66) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IGF1R Antibody (N-term K66) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (IGF1R Antibody (N-term K66) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Immunofluorescence (IF) (Confocal immunofluorescent analysis of IGF1R Antibody (N-term K66) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
The IGF1R receptor binds insulin-like growth factor with a high affinity and plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. The protein possess tyrosine kinase activity.