anti-IL-33 antibody product blog
Tags: Antibody; Polyclonal Antibody; IL-33; anti-IL-33 antibody;
The IL-33 il33 (Catalog #MBS695288) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Biotinylated Anti-hIL-33 reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s IL-33 can be used in a range of immunoassay formats including, but not limited to, Optimal dilutions should be determined by each laboratory for each application.Direct ELISA: To detect Human IL-33 by direct ELISA (using 100 ul/well) a concentration of approximately 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody allows the detection of at least 2000-4000 pg/ml of recombinant Human IL-33.
Sandwich ELISA: To detect Human IL-33 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 - 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IL-33 (MBS695225) as a capture antibody, allows the detection of at least 2000-4000 pg/ml of recombinant hIL-33.
Western Blot: To detect hIL-33 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-33 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Researchers should empirically determine the suitability of the IL-33 il33 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The IL-33 il33 product has the following accession number(s) (GI #68565855) (NCBI Accession #O95760.1) (Uniprot Accession #O95760). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-33 (human IL-33). Anti-Human IL-33 specific antibody was purified by affinity chromatography and then biotinylated.
Immunogen: E.coli derived recombinant Human IL-33 (MBS695839)
Preparation: Produced from sera of rabbits immunized with highly pure recombinant Human IL-33 specific antibody was purified by affinity chromatography and then biotinylated.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. . In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing IL-33 are readily searchable from our website. Different antibodies against the same target such as IL-33 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.