anti-INS antibody product blog
Tags: Antibody; INS; Polyclonal Antibody; anti-INS antibody;
The INS ins (Catalog #MBS127210) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The INS Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s INS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).WB: 1:500 - 1:2000
IHC: 1:50 - 1:200. Researchers should empirically determine the suitability of the INS ins for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The INS ins product has the following accession number(s) (GI #124617) (NCBI Accession #P01308.1) (Uniprot Accession #P01308). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the INS Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of mouse pancreas, using INS antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.)
The maintenance of glucose homeostasis is an essential physiological process that is regulated by hormones. An elevation in blood glucose levels during feeding stimulates insulin release from pancreatic beta cells through a glucose sensing pathway (1). Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Insulin stimulates glucose uptake from blood into skeletal muscle and adipose tissue. Insulin deficiency leads to type 1 diabetes mellitus (2).
Source: Rabbit
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human INS
Calculated Molecular Weight: 12kDa
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing INS are readily searchable from our website. Different antibodies against the same target such as INS may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. INS also interacts with the following gene(s): AKT1, AKT2, FOXO1, GRB2, IDE, IGFBP1, INSR, IRS1, IRS2, PTPN1. Diabetes Mellitus, Diabetes Mellitus, Type 2, Fatty Liver, Hyperglycemia, Hyperinsulinism, Hypertension, Insulin Resistance, Metabolic Diseases, Obesity, Polycystic Ovary Syndrome are some of the diseases may be linked to INS Polyclonal Antibody.