anti-IRF5 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-IRF5 antibody; IRF5;
The IRF5 irf5 (Catalog #MBS2529606) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IRF5 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s IRF5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).WB: 1:500-1:2000,IHC: 1:50-1:200,IF :1:50-1:200. Researchers should empirically determine the suitability of the IRF5 irf5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The IRF5 irf5 product has the following accession number(s) (GI #334883190) (NCBI Accession #NP_001229381.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the IRF5 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using IRF5 antibody.)
Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cell using IRF5 antibody. Blue: DAPI for nuclear staining.)
Background/Introduction: Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3gamma/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2).IRF-5 is expressed in lymphoid tissues and peripheral blood lymphocytes and participates in the induction of type I interferon genes following viral infection (3). Activation of IRF-5 signaling is triggered by the toll-like receptor (TLR) pathway, including TLR7 and MyD88 (4,5). Genetic variants of IRF-5 have been associated with disorders where the IFN pathway is abnormally activated, such as systemic lupus erythematosus (6,7).
Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-98651. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing IRF5 are readily searchable from our website. Different antibodies against the same target such as IRF5 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.