|The IRS1 irs1 (Catalog #MBS617982) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IRS-1 pre-CT (Insulin Receptor Substrate 1) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s IRS-1 pre-CT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP).
Suitable for use in Western Blot and Immunoprecipitation.
Dilution: Western Blot: 0.5-2ug/ml detected IRS-1 in a 3T3/A31 RIPA cell lysate.
Immunoprecipitation: 4ug immunoprecipitated IRS-1 from 500ug of a 3T3/A31 RIPA lysate.
Optimal dilution determined by the researcher. Researchers should empirically determine the suitability of the IRS1 irs1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The IRS1 irs1 product has the following accession number(s) (GI #5031805) (NCBI Accession #NP_005535.1) (Uniprot Accession #P35568). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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The insulin receptor substrate 1 (IRS1) is a major effector of insulin receptor action. Unlike other activated receptors (e.g., the EGF receptor and the PDGF receptor) which directly bind to multiple signalling proteins, the activated insulin receptor primarily binds only IRS1 and then phosphorylates it on multiple tyrosine residues. Phosphorylated IRS1 serves as the major agent of insulin receptor-mediated signal transduction by being bound by cytoplasmic signaling proteins containing SH2 domains. Numerous SH2-containing signaling proteins (e.g., Grb2, SH-PTP2/SYP, PI 3-kinase, and Nck) have been shown to preferentially bind to distinct tyrosine phosphorylation sites in IRS1. The specificity and coordination of insulin receptor signaling may result from competition of these signaling proteins for binding to IRS1.
Immunogen: Precarboxy-terminal peptide (16aa) corresponding to aa1220-1235 of rat liver IRS-1 (KLH coupled). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing IRS1 are readily searchable from our website. Different antibodies against the same target such as IRS1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Bone, Brain, Connective Tissue, Heart, Kidney, Liver, Muscle, Nerve, Ovary, Pancreas tissues are correlated with this protein. IRS1 also interacts with the following gene(s): AKT1, GRB2, IGF1R, INSR, JAK2, MAPK1, MTOR, PTPN11, SHC1, SOCS3.