anti-IRS4 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-IRS4 antibody; IRS4; Insulin-Like Growth Factor I Receptor/Insulin Receptor;
The IRS4 irs4 (Catalog #MBS612046) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Insulin-Like Growth Factor I Receptor/Insulin Receptor, phosphorylated (Tyr1131/Tyr1158) (IGF1R, IR) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Insulin-Like Growth Factor I Receptor/Insulin Receptor can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the IRS4 irs4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The IRS4 irs4 product has the following accession number(s) (GI #4504733) (NCBI Accession #NP_003595.1) (Uniprot Accession #O14654). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Insulin-Like Growth Factor I Receptor/Insulin Receptor, phosphorylated (Tyr1131/Tyr1158) (IGF1R, IR)
Biological actions of insulin and IGF1 are mediated by their respective cell surface receptors, both of which are receptor tyrosine kinases that regulate multiple signaling pathways through activation of a series of phosphorylation cascades. The IR and IGF1R are heterotetrameric proteins consisting of two ligand-binding a subunits and two b subunits that each contain a tyrosine kinase domain. Insulin/IGF1 binding to the extracellular domain leads to autophosphorylation of the receptor and activation of the intrinsic tyrosine kinase activity, which allows appropriate substrates to be phosphorylated. These two receptors differ in sequence in regions that confer specificity for the designated ligand as well as in certain intracellular signaling domains. These differences allow insulin and IGF-1 to regulate different physiological functions through receptors that share a very similar structure. Phosphorylation sites that are unique to each receptor presumably play a key role in these signaling differences. The catalytic loops within the tyrosine kinase domains of the IR/IGF1R contain a three tyrosine motif corresponding to Tyr1158, 1162 and 1163 (for the IR) and Tyr1131, 1135 and 1136 (for the IGF1R).
Immunogen: Synthetic phosphopeptide corresponding to IR/IGF1R that contains tyrosine 1158 of the human insulin receptor (IR) The corresponding residue in the IGF1R is 1131. Species sequence homology: mouse and rat for both the IR and IGF1R. Positive Control: CHO-T cells transfected with a vector containing insulin receptor and stimulated with insulin, and
HepG2 cells +/- insulin. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing IRS4 are readily searchable from our website. Different antibodies against the same target such as IRS4 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.