anti-IRS4 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-IRS4 antibody; IRS4; Insulin-Like Growth Factor I Receptor/Insulin Receptor;
The IRS4 irs4 (Catalog #MBS616009) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Insulin-Like Growth Factor I Receptor/Insulin Receptor, phosphorylated (Tyr1158, Tyr1162, Tyr1163) (IGF1R, IR) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Insulin-Like Growth Factor I Receptor/Insulin Receptor can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the IRS4 irs4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The IRS4 irs4 product has the following accession number(s) (GI #4504733) (NCBI Accession #NP_003595.1) (Uniprot Accession #O14654). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Insulin-Like Growth Factor I Receptor/Insulin Receptor, phosphorylated (Tyr1158, Tyr1162, Tyr1163) (IGF1R, IR) with the following immunoassay(s):
Testing Data (Untreated (Lane 1) or insulin treated (Lanes 2, 3, and 4) lysates from CHO cells transfected with IR were resolved by electrophoresis, transferred to nitrocellulose, and probed with MBS616009 for 2 hours at room temperature. Lane 3 was pre-incubated with the blocking peptide immunogen corresponding to the IR (Tyr1162/Tyr1163). Lane 4 was pre-incubated with the blocking peptide immunogen corresponding to phospho-IR (Tyr1158/Tyr1162/Tyr1163). Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-IR.)
Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane tyrosine kinase. It is widely expressed among many cell types in fetal and postnatal tissues and many cell lines. Upon binding of its ligands, IGF-I and IGF-II, autophosphorylation of receptors occurs. The triple tyrosine cluster (Tyr1131, Tyr1135 and Tyr1136) within the kinase domain is the earliest major site of autophosphorylation. Phosphorylation of these three tyrosine residues is necessary for activation of kinases. Insulin receptors (IRs) share significant similarity with IGF-I receptors in structure and function. There is also an equivalent triple tyrosine cluster (Tyr1146, Tyr1150 and Tyr1151) in its activation loop of the kinase domain. Tyrosine autophosphorylation of the receptors is one of the earliest cellular responses to insulin stimulation. The autophosphorylation begins by phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151. The full activation of receptor kinase activity depends on the triple tyrosine phosphorylation.
Immunogen: Peptide corresponding to amino acid region encompassing the human, mouse and rat phospho-IR/IGF1R (Tyr1158/Tyr1162/Tyr1163). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing IRS4 are readily searchable from our website. Different antibodies against the same target such as IRS4 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.