anti-KEAP1 antibody product blog
Tags: Antibody; Polyclonal Antibody; Keap1; anti-KEAP1 antibody;
The KEAP1 keap1 (Catalog #MBS247045) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Polyclonal to Human KEAP1 reacts with Bat, Gibbon, Bovine, Dog, Gorilla, Horse, Human, Monkey, Orangutan, PigPredicted Reactivity: Mouse, Rat, Hamster (at least 90% immunogen sequence identity) and may cross-react with other species as described in the data sheet. MyBioSource\'s KEAP1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA).
ELISA (1:32000), IHC-P (3.75 ug/ml), WB (0.2 - 0.6 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 75kD band observed in lysates of cell lines HeLa and NIH3T3 (calculated MW of 69.7kD according to Human NP_036421.2 and of 69.6kD according to Mouse NP_057888.1). Recommen.. Researchers should empirically determine the suitability of the KEAP1 keap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The KEAP1 keap1 product has the following accession number(s) (GI #22027642) (NCBI Accession #NP_036421.2) (Uniprot Accession #Q14145). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat Polyclonal to Human KEAP1 with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-KEAP1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.)
Western Blot (WB) (KEAP1 antibody (0.2 ug/ml) staining of NIH3T3 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Target Species: Human
Immunogen Description: Synthetic peptide C-EVTPSQHGNRTFS from an internal region (near N-terminus) of human KEAP1 (NP_036421.2). Immunogen Type: Synthetic peptide
Immunogen: KEAP1 antibody was raised against synthetic peptide C-EVTPSQHGNRTFS from an internal region (near N-terminus) of human KEAP1 (NP_036421.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum (100%); Mouse, Rat, Hamster (92%); Lizard (85%).
Antigen Modification: N-Terminus. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing KEAP1 are readily searchable from our website. Different antibodies against the same target such as KEAP1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. KEAP1 also interacts with the following gene(s): CUL3, NFE2L2, PGAM5, RBX1, RELA, SQSTM1, UBC.