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anti-KLF2 antibody product blog

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Posted on 2015-10-28 02:47:59 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; anti-KLF2 antibody; KLF2;
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The KLF2 klf2 (Catalog #MBS176139) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-KLF2 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s KLF2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the KLF2 klf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The KLF2 klf2 product has the following accession number(s) (GI #341940872) (NCBI Accession #Q60843.2) (Uniprot Accession #Q60843). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-KLF2 antibody with the following immunoassay(s):
Western Blot (WB) (Anti-KLF2 antibody, MBS176139, Western blotting
Lane 1: Recombinant Human KLF2 Protein 10ng
Lane 2: Recombinant Human KLF2 Protein 5ng
)
Western Blot (WB) KLF2.

Description: Rabbit IgG polyclonal antibody for Krueppel-like factor 2(KLF2) detection. Tested with WB in Human, Mouse, Rat.
Background: KLF2(Krueppel-like factor 2), also known as LKLF(LUNG KRUPPEL-LIKE ZINC FINGER TRANSCRIPTION FACTOR), is a protein that in humans is encoded by the KLF2 gene. Kruppel-like factors(KLFs) are a family of broadly expressed zinc finger transcription factors. KLF2 regulates T-cell trafficking by promoting expression of the lipid-binding receptor S1P1(S1PR1) and the selectin CD62L. Kozyrev et al.(1999) mapped the KLF2 gene to chromosome 19p13.13-p13.11, close to the KLF1 gene, by radiation hybrid analysis. Wani et al.(1999) mapped the KLF2 gene to 19p13.1 by somatic cell hybrid analysis and FISH. Buckley et al.(2001) showed that expression of LKLF in a T-cell line induced reversible quiescence resulting in marked inhibition of proliferation, decreases in cell size and protein synthesis, and reduced expression of activation markers. They concluded that LKLF functions, in part, by negatively regulating a MYC-dependent pathway.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of mouse KLF2 (19-32aa ERGLQERWPRNEPE), identical to the related rat sequence and different from the related human sequence by one amino acid. Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing KLF2 are readily searchable from our website. Different antibodies against the same target such as KLF2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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