anti-KRT10 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-KRT10 antibody; KRT10;
The KRT10 krt10 (Catalog #MBS631114) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KRT10, NT (Keratin, Type I Cytoskeletal 10, Cytokeratin-10, CK-10, Keratin-10, K10, KPP) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s KRT10 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC).Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100. Researchers should empirically determine the suitability of the KRT10 krt10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The KRT10 krt10 product has the following accession number(s) (GI #195972866) (NCBI Accession #NP_000412.3) (Uniprot Accession #P13645). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the KRT10, NT (Keratin, Type I Cytoskeletal 10, Cytokeratin-10, CK-10, Keratin-10, K10, KPP) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of KRT10 Antibody (N-term) in 293 cell line lysates (35ug/lane). KRT10 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human kidney carcinoma with KRT10 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (Flow cytometric analysis of 293 cells using KRT10 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
KRT10 is a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in its gene are associated with epidermolytic hyperkeratosis.
Immunogen: Synthetic peptide selected from the N-terminal region of human KRT10 (KLH). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing KRT10 are readily searchable from our website. Different antibodies against the same target such as KRT10 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.