Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Polyclonal Antibody Blog  rightarrow anti-KRT18 antibody  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • anti-ETFA antibody blog
 • anti-RING1 antibody blog
 • anti-LEPREL4 antibody blog
 • anti-ACBD3 antibody blog
 • anti-RNASE6 antibody blog
 • anti-cRAF antibody blog
 • anti-PLA2G12A antibody blog
 • anti-TBC1D28 antibody blog
 • anti-CD24 antibody blog
 • anti-WFDC2 antibody blog
 • anti-AGRP antibody blog
 • anti-CD83 antibody blog
 • anti-SHOX2 antibody blog
 • anti-NFIX antibody blog
 • anti-Neisseria gonorrhoeae antibody blog
 • anti-ATP5A1 antibody blog
 • anti-ARF1 antibody blog
 • anti-LIN28B antibody blog
 • anti-DUSP14 antibody blog
 • anti-TNFRSF19 antibody blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • Histone H2B (HIST1H2BB) Antibody
 • IL-16 Antibody
 • CUL3 Antibody
 • STAT6 Antibody
 • 53BP1 Antibody
 • HK1 Antibody
 • Fucosyltransferase 2 (FUT2) ELISA Kit
 • CDCA8 Antibody
 downarrow more ...
separator

anti-KRT18 antibody product blog

separator
Posted on 2018-05-28 15:08:05 by mybiosource_staff
Share it on ...     
separator
separator
Tags: Antibody; Polyclonal Antibody; anti-KRT18 antibody; KRT18; Keratin, type I cytoskeletal 18;
separator
The KRT18 krt18 (Catalog #MBS7000063) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Human Keratin, type I cytoskeletal 18 polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Keratin, type I cytoskeletal 18 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC).
Recommended dilution: WB:1:500-2000, IHC:1:20-1:200. Researchers should empirically determine the suitability of the KRT18 krt18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The KRT18 krt18 product has the following accession number(s) (GI #4557888) (NCBI Accession #NP_000215.1) (Uniprot Accession #P05783). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit anti-Human Keratin, type I cytoskeletal 18 polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot
All lanes: Keratin, type I cytoskeletal 18 antibody at 2ug/ml
Lane 1: K562 whole cell lysate
Lane 2: HepG2 whole cell lysate
Secondary
Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 48kDa
Observed band size: 48kDa
)
Western Blot (WB) KRT18.

Western Blot (WB) (Western blot All lanes: KRT18 antibody at 12ug/ml
Lane 1: A431 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: Jurkat whole cell lysate
Lane 4: Zebrafish lysate
Secondary
Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 48 kDa
Observed band size: 48,66 kDa
)
Western Blot (WB) KRT18.

Western Blot (WB) (Western Blot
Positive WB detected in: K562 whole cell lysate,Hela whole cell lysate
All lanes: KRT18 antibody at 3ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49 KDa
Observed band size: 49 KDa
)
Western Blot (WB) KRT18.

Immunohistochemistry (IHC) (IHC image of MBS7000063 diluted at 1:800 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC) KRT18.

Immunofluorescence (IF) (Immunofluorescent analysis of HepG2 cells using MBS7000063 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunofluorescence (IF) KRT18.

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells By similarity. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.

horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions