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anti-LC3B antibody product blog

Posted on 2016-09-07 16:03:04 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; LC3B; anti-LC3B antibody;
The LC3B n/a (Catalog #MBS6008414) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LC3B, phosphorylated (T93) (MAP1LC3B, LC3B, MAP1A/1BLC3, MAP1LC3B) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s LC3B can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Gel Shift Assay (GS/EMSA).
Suitable for use in Dot Blot, ELISA
Dilution: ELISA: 1:1,000
Dot Blot 1:500. Researchers should empirically determine the suitability of the LC3B n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3b is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).

LC3B n/a

Immunogen: Phospho-LC3B-T93 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 83~112 amino acids surrounding T93 of human MAP1LC3B. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing LC3B are readily searchable from our website. Different antibodies against the same target such as LC3B may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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