|The LEFTY1 lefty1 (Catalog #MBS2004675) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Left/Right Determination Factor 1 (LEFTY1) reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Left/Right Determination Factor 1 (LEFTY1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB).
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the LEFTY1 lefty1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The LEFTY1 lefty1 product has the following accession number(s) (GI #6753702) (NCBI Accession #NP_034224.1) (Uniprot Accession #Q64280). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF -SI HESGWKAFDV TEAVNFWQQL SRPRQPLLLQ VSVQREHLGP GTWSSHKLVR FAAQGTPDGK GQGEPQLELH TLDLKDYGAQ GNCDPEAPVT EGTRCCRQEM YLDLQGMKWA ENWILEPPGF LTYECVGSCL QLPESLTSRW PFLGPRQCVA SEMTSLPMIV SVKEGGRTRP QVVSLPNMRV QTCSCASDGA LIPRRLQP.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Polyclonal Antibody to Left/Right Determination Factor 1 (LEFTY1) with the following immunoassay(s):
Western Blot (WB) ((Figure. Western Blot; Sample: Recombinant LEFTY1, Mouse.))
Immunohistochemistry (IHC) ((Figure.DAB staining on IHC-P. Samples: Mouse Tissue))
Immunogen (Antigen): Recombinant LEFTY1 (Ser169~Pro368) expressed in E.coli. Immunogen (Antigen): Recombinant LEFTY1 (Ser169~Pro368) expressed in E.coli. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing LEFTY1 are readily searchable from our website. Different antibodies against the same target such as LEFTY1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. LEFTY1 also interacts with the following gene(s): Cfc1, Nodal, Pou5f1, Tdgf1, Tgfb1. Brain, Embryonic Tissue, Intestine, Liver, Lung, Pancreas, Skin, Spleen, Testis tissues are correlated with this protein.