anti-Lep antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-lep antibody; lep; Leptin [131-145];
The Lep lep (Catalog #MBS352143) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Leptin [131-145] can be used in a range of immunoassay formats including, but not limited to, Immunocytochemical Staining, Western Immunoblot, Western Blot.Immunocytochemistry: The antisera have been found to stain fixed adipocytes known to express leptin using ABC techniques at a dilution of 1:800
Western Blot: Western immunoblots using adipocyte homogenates have been successful at a dilution of 1:500 and yield a single band at ~ 16 kDa.
Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 4% normal goat serum in TBS/Tween-20 buffer.
2. Wash x 2 with TBS/Tween-20.
3. Apply the rabbit polyclonal antibody after dilution to at least 1:500 (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20. Let the primary antibody bind for 2-4 hours.
4. Wash x 3 with TBS/Tween-20.
5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.
6. Wash for 5 minutes x 4 in TBS/Tween-20.
7. Develop color using the enhanced DAB reaction. Researchers should empirically determine the suitability of the Lep lep for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Lep lep product has the following accession number(s) (GI #6981148) (NCBI Accession #NP_037208.1) (Uniprot Accession #P50596). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
The pooled antisera were raised in rabbits which were immunized with a peptide analogue of the carboxyl terminal of human leptin covalently attached onto a carrier protein. The pooled antisera are specific for the COOH terminal of leptin and are suitable for immunocytochemistry and western immunoblotting. The pooled antisera have been characterized by western blotting. ELISA. RIA and cell staining techniques. Dilute the lyophilized antisera with 0.1 ml of 10 mg/ml BSA is PBS for the equivalent of whole pooled antisera. or with additional buffer for more dilute antisera.
In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Lep are readily searchable from our website. Different antibodies against the same target such as Lep may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.