anti-LEP antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-lep antibody; lep;
The LEP lep (Catalog #MBS9201815) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LEP Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. MyBioSource\'s LEP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS).WB~~1:1000. Researchers should empirically determine the suitability of the LEP lep for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The LEP lep product has the following accession number(s) (GI #4557715) (NCBI Accession #NP_000221.1) (Uniprot Accession #P41159). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the LEP Antibody (N-term) with the following immunoassay(s):
Western Blot (WB) (LEP Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the LEP antibody detected the LEP protein (arrow).)
Western Blot (WB) (Western blot analysis of LEP (arrow) using rabbit polyclonal LEP Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the LEP gene.)
Immunofluorescence (IF) (Confocal immunofluorescent analysis of LEP Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Immunohistochemistry (IHC) (LEP Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LEP Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (LEP Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
This gene encodes a protein that is secreted by white
adipocytes, and which plays a major role in the regulation of body
weight. This protein, which acts through the leptin receptor,
functions as part of a signaling pathway that can inhibit food
intake and/or regulate energy expenditure to maintain constancy of
the adipose mass. This protein also has several endocrine
functions, and is involved in the regulation of immune and
inflammatory responses, hematopoiesis, angiogenesis and wound
healing. Mutations in this gene and/or its regulatory regions cause
severe obesity, and morbid obesity with hypogonadism. This gene has
also been linked to type 2 diabetes mellitus development. [provided
by RefSeq].
Antigen Type: Synthetic Peptide. Antigen Source: HUMAN. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing LEP are readily searchable from our website. Different antibodies against the same target such as LEP may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.