|The LIMK1 limk1 (Catalog #MBS472032) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LIMK1 (C-terminus) reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s LIMK1 (C-terminus) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot.
Western Blot: 1:1000. Researchers should empirically determine the suitability of the LIMK1 limk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The LIMK1 limk1 product has the following accession number(s) (GI #4505001) (NCBI Accession #NP_002305.1) (Uniprot Accession #P53667). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the LIMK1 (C-terminus) with the following immunoassay(s):
LIM kinases (LIMK1 and LIMK2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs, in their amino-terminal
regulatory domains. LIM kinases are involved in actin cytoskeletal regulation downstream of Rho-family GTPases, PAKs, and ROCK. PAK1
and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr-508 or Thr-505 residues in the activation loop, increasing LIMK activity. In addition, VEGF-induced stress fiber formation has been linked to p38- mediated activation of LIMK through MK-2 phosphorylation of Ser-323. Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the amino-terminal Ser-3 residue of cofilin. In addition, LIMKs may have a function in the nucleus. It has been shown that the nuclear localization of LIMKs can mediate suppression of Rac/Cdc42-mediated cyclin D1 expression. This effect of LIMKs was independent of cofilin phosphorylation and the regulation of actin dynamics.
Immunogen: LIMK1 (C-terminus) synthetic peptide (coupled to carrier protein) corresponding to amino acids at the C-terminus of human LIMK1. This
sequence is conserved in rat and mouse LIMK1, and is not found in LIMK2. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing LIMK1 are readily searchable from our website. Different antibodies against the same target such as LIMK1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene.