anti-LIMPII antibody product blog
Tags: Antibody; Polyclonal Antibody; LIMPII / SCARB2; anti-LIMPII antibody; LIMPII;
The LIMPII scarb2 (Catalog #MBS244098) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Polyclonal to Human LIMPII / SCARB2 reacts with Gibbon, Gorilla, Human, RabbitPredicted Reactivity: Monkey, Mouse, Hamster, Pig (at least 90% immunogen sequence identity) and may cross-react with other species as described in the data sheet. MyBioSource\'s LIMPII / SCARB2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA).
ELISA (1:4000), IHC-P (3.75 ug/ml), WB (0.1 - 0.3 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Approx. 80kDa band observed in Human Brain (Cortex and Cerebellum) lysates (calculated MW of 54.3kDa according to NP_005497.1). The observed molecular weight corresponds to earlie.. Researchers should empirically determine the suitability of the LIMPII scarb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The LIMPII scarb2 product has the following accession number(s) (GI #5031631) (NCBI Accession #NP_005497.1) (Uniprot Accession #Q14108). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat Polyclonal to Human LIMPII / SCARB2 with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-LIMPII / SCARB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.)
Western Blot (WB) (SCARB2 antibody (0.1 ug/ml) staining of Human Cerebral Cortex lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Target Species: Human
Immunogen Description: Synthetic peptide C-NKANIQFGDNGTTIS from an internal region of human SCARB2 / SR-BII (NP_005497.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Rabbit (100%); Monkey, Mouse, Hamster, Elephant, Panda, Pig (93%). Immunogen Type: Synthetic peptide
Immunogen: LIMPII / SCARB2 antibody was raised against synthetic peptide C-NKANIQFGDNGTTIS from an internal region of human SCARB2 / SR-BII (NP_005497.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Rabbit (100%); Monkey, Mouse, Hamster, Elephant, Panda, Pig (93%); Rat, Bovine, Dog, Bat (87%); Horse (80%).
Antigen Modification: Internal. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing LIMPII are readily searchable from our website. Different antibodies against the same target such as LIMPII may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.