anti-LIPE antibody product blog
Tags: Antibody; Hormone Sensitive Lipase; Polyclonal Antibody; LIPE; anti-LIPE antibody;
The LIPE lipe (Catalog #MBS616664) is an Antibody produced from Chicken and is intended for research purposes only. The product is available for immediate purchase. The Hormone Sensitive Lipase (HSL) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Hormone Sensitive Lipase can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).Suitable for use in Western Blot and ELISA.
Dilution: ELISA: 1:1000-1:5000
Western Blot: 1:1000-1:5000. Researchers should empirically determine the suitability of the LIPE lipe for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The LIPE lipe product has the following accession number(s) (GI #21328446) (NCBI Accession #NP_005348.2) (Uniprot Accession #Q05469). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Lipase, hormone-sensitive also known as LIPE is an enzyme that, in humans, is encoded by the LIPE gene. LIPE is an intracellular neutral lipase that is capable of hydrolyzing a variety of esters.[2] The enzyme has a long and a short form. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids.
LIPE functions to hydrolyze the first fatty acid from a triacylglycerol molecule, freeing a fatty acid and diglyceride. It is also known as triglyceride lipase, while the enzyme that cleaves the second fatty acid in the triglyceride is known as diglyceride lipase, and the third enzyme that cleaves the final fatty acid is called monoglyceride lipase. Only the initial enzyme is affected by hormones, hence its hormone-sensitive lipase name. The diglyceride and monoglyceride enzymes are tens to hundreds of times faster, hence HSL is the rate-limiting step in cleaving fatty acids from the triglyceride molecule.
LIPE is activated when the body needs to mobilize energy stores, and so responds positively to catecholamines, ACTH, and negatively to insulin. Previously, glucagon was thought to activate LIPE, however the removal of insulin\'s inhibitory effects ("cutting the brakes") is the source of activation. The lipolytic effect of glucagon in adipose tissue is minimal in humans. LIPE may be activated by two mechanisms. In the first, phosphorylated perilipin A causes it to move to the surface of the lipid droplet, where it may begin hydrolyzing the lipid droplet. Also, it may be activated by a cAMP-dependent protein kinase (PKA). This pathway is significantly less effective than the first, which is necessary to lipid mobilization in response to cyclic AMP, which itself is provided by beta adrenergic stimulation of the glucagon receptor.