anti-LITAF antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-LITAF antibody; LITAF;
The LITAF litaf (Catalog #MBS9126594) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LITAF Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s LITAF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200. Researchers should empirically determine the suitability of the LITAF litaf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The LITAF litaf product has the following accession number(s) (GI #83304387) (NCBI Accession #Q99732.2) (Uniprot Accession #Q99732). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the LITAF Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using LITAF antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human prostate using LITAF Antibody at dilution of 1:100 (40x lens).)
Lipopolysaccharide is a potent stimulator of monocytes and macrophages, causing secretion of tumor necrosis factor-alpha (TNF-alpha) and other inflammatory mediators. This gene encodes lipopolysaccharide-induced TNF-alpha factor, which is a DNA-binding protein and can mediate the TNF-alpha expression by direct binding to the promoter region of the TNF-alpha gene. The transcription of this gene is induced by tumor suppresor p53 and has been implicated in the p53-induced apoptotic pathway. Mutations in this gene cause Charcot-Marie-Tooth disease type 1C (CMT1C) and may be involved in the carcinogenesis of extramammary Paget\'s disease (EMPD). Multiple alternatively spliced transcript variants have been found for this gene.
Species: Human
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human LITAF
Calculated Molecular Weight: 17kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing LITAF are readily searchable from our website. Different antibodies against the same target such as LITAF may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Bone, Heart, Intestine, Kidney, Liver, Lung, Muscle, Nerve, Spleen tissues are correlated with this protein.